Coding

Part:BBa_K1774001:Design

Designed by: HKU iGEM 2015   Group: iGEM15_Hong_Kong_HKU   (2015-09-18)


Cas9 (optimized for expression in E. coli)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3512


Design Notes

Presence of illegal RE sites


Source

S. pyogenes SF 370

References